Fluorescence intensity (530/585 nm) is linear to the bile acid concentration in the sample. BIOCRATES Life Sciences AG operates as a research and technology. Bile acid is metabolized in the liver and is present in increased concentrations with abnormal liver function. The kit is designed to analyze 16 individual human and 19 mouse bile acids. The BIOCRATES® Bile Acids Kit* is based on (ultra) high performance liquid chromatography coupled with electrospray tandem mass spectrometry (UHPLC-MS/MS) and is characterized by quick and highly effective sample extraction on a 96-well patented filter plate. Samples were prepared using the Bile Acids kit (Biocrates Innsbruck, Austria) in strict accordance with their detailed protocol. Fecal BAs were assessed by electrospray (ESI) mass spectrometry coupled to liquid chromatography (LC) with a tandem mass spectrometry (MS/MS) instrument (AB SCIEX, ThermoScientific, Waters) using a kit customized by Biocrates® (Biocrates® Bile Acids Kit), which enabled the absolute quantification of up to 20 different BA. Liquid handling is done on ML Star Hamilton robot. Total bile acid (TBA) level was calculated by summation of 15 conjugated and unconjugated primary and secondary BA levels. Laboratories were first required to pass the acceptance criteria for the SST. locale: session: 1 month: Used by the site to show content to users based on their selected location. Acute heart failure is characterized by a heavily reorganized metabolic phenotype especially in amino acids and lipids in … A standardized quantitative Kit developed that allows the simultaneous analysis of 20 bile acids in 20 μL sample volume. Detection range: 1 - 150 µM bile acids. Bile acid quantification was performed by the Sick Kids Analytical Facility for Molecules (AFBM) by liquid chromatography tandem mass spectrometry (LC-MS-MS) using the Biocrates® Life Sciences Bile Acids Kit (Biocrates, Innsbruck, Austria) according to the manufacturer’s instructions. spectrometer (Waters Inc., Milford, Massachusetts, USA). The novel Bile Acid kit developed by Biocrates allows to measure 20 bile acid metabolites with very high sensitivity (up to 3 nM). An injection volume of 10 μL was used. Each laboratory received a Biocrates ® Bile Acids Kit including system suitability test (SST) protocol. Bile acids also exist as sulfate salt forms known as bile acid sulfates. The nomeclature has been proposed by Hofmann et al. Quantification of 20 steroids is done using Biocrates AbsoluteIDQTM technology. We also used Biocrates AbsoluteIDQ® p150 kit (BIOCRATES Life Sciences AG, . Bile acids are an important test for monitoring normal liver function. Bile acids (BAs), products of cholesterol metabolism and clearance, are produced in the … Introduction: Increasing evidence suggests a role for the gut microbiome in central nervous system disorders and a specific role for the gut-brain axis in neurodegeneration. BIOCRATES Life Sciences, an Austrian developer of mass spectrometry-based kits, has launched the first ever LC-MS assay for determining endogenous bile acids. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion. Cell Biolabs’ Total Bile Acid Assay Kit is a simple colorimetric assay that measures the amount of total bile acid present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. Biocrates ® Bile Acids Kit - primary bile acids - secondary bile acids - human and rodent specific bile acids. View Hartmut Glossmann’s professional profile on Relationship Science, the database of decision makers. 6. Bile Acid Kit (20) (Kit / Service) Bile acids are important indicators of the hepato-biliary function as well as the gut microbiome. 188 metabolites, which were ca-refully selected based on studies of the human blood metabolome. It provides metabolic phenotyping services. The kit has been validated according to the EMA guidelines for human and rat plasma. Quantification of bile acids in both brain tissue extract and plasma were performed using the Biocrates Bile Acids kit (BIOCRATES, Life Science AG, Innsbruck, Austria). The lysine degradation product 2-aminoadipic acid is heavily decreased in concentration which already hints at a protective effect according to the authors. App Note. . BA quantification was performed by liquid chromatography tandem mass spectrometry using the Biocrates Life Sciences Bile Acids Kit (BIOCRATES Life Science AG, Innsbruck, Austria) according to manufacturer's instructions (see Table 3 for list of BAs, abbreviations, and their levels across diagnosis groups). BioAssay Systems' Bile Acid Assay Kit (EFBA-100) provides a fluorimetric means to measure total bile acids. Objectives: The aim of the present study is to measure concentrations of 20 bile acids using the novel Kit from Biocrates Life Sciences based on LC-MS technique. The firm products are absoluteIDQ p150 kit, p180 kit, sterio17 kit, steroid reagents, biocrates bile acids kit, and steroid kit. Bile Acid quantification – Biocrates AbsoluteIDQ - Bile Acids Kit. The bile acid kit provides simultaneous quantification of 22 bile acids (properties outlined in Table 6). Assays are based on LC-MS/MS separation and detection (Agilent … Kairos Amino Acid A set of 9 human and mouse plasma samples was measured in replicates. The microbiota induces modifi cations (dehydroxylation and/or decon- The complete analytical process (Data processing, sample registration, work list generation) was accomplished using the MetIDQ™ software package, which is an integral part of the Biocrates ® Bile Acid Kit. This assay can be used to measure bile acid concentrations in mice in a wide variety of applications including mouse serum, liver, bile, feces, and intestine. BIOCRATES Life Sciences AG, developer of cutting-edge mass-spectrometry-based kit products, has launched the first-ever bile acids kit based on targeted metabolite quantitation. Their signaling functions play an important role in chronic disorders, sepsis, or The major bile acid is cholic acid, which can be conjugated or unconjugated forming different isoforms: deoxy-glyco-cheno-lith-hyo-tauro or urso. The MxP Quant 500 kit combines LC-MS/MS of 13 compound classes, basically small molecules, bile acids, and free fatty acids, followed by FIA-MS/MS of 12 lipid classes (including acylcarnitines) and hexoses, into a single workflow (Figure 1). Objectives The aim of the present study is to measure concentrations of 20 bile acids using the novel Kit from Biocrates Life Sciences based on LC-MS technique. Metabolomics (Bile Acids) Created By Mette Peters Mette The objective was to measure the levels of selected bile acids in 159 human plasma and serum samples using the Biocrates Bile Acids kit. Cholic acid AbsoluteIDQ p180 (188) (Kit / Service) Our most frequently used targeted metabolomics discovery panel. Samples were processed according to the manufacturer’s instructions. Samples: serum, plasma, urine and other biological samples. Higher serum bile acid levels and an altered gut microbiome contribute to fibrogenesis, liver injury, and tumorigenesis in cirrhotic and noncirrhotic NASH-HCC. The Biocrates bile acid panel measures the concentration of 20 different bile acids. Cookie Type Duration Description; fpestid: 0: 1 year: Fpestid is a ShareThis cookie ID set in the domain of the website operator. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples. The Biocrates® Bile Acids Kit is the fi rst ready to use solution to quantify 20 bile acids in a standardized and high throughput fashion. [16]. Biocrates Bile Acids. … The synthesis of primary bile acids, and the conjugation of bile acids with tauring and glycine, takes place in the liver. Read user reviews, compare products & request pricing of BIOCRATES Life Sciences AG - Cell-Based Assays laboratory products & instrumentation. The Biocrates Bile Acids assay quantifies 20 bile acids, 16 of which are normally detected in human plasma. Addition of 10 µL of the supplied internal standard solution to each well of the 96-well extraction plate was followed by drying under a gentle stream of nitrogen. Bile acids are essential for regulating cholesterol metabolism and the intestinal digestion and absorption of fat. Austrian BIOCRATES Life Sciences AG, internationally renowned developer and marketer of innovative targeted metabolomics solutions, further expands its range of metabolomics-based Biocrates© Bile Acids Kit (96 wells) - Waters for up to 84 samples; for HPLC and UHPLC pump System Appelez-nous : +33 (0)2.32.64.45.45 Liste des fabricants Site instrumentation Hartmut Glossmann is Co-Founder at BIOCRATES Life Sciences AG. BIOCRATES Life Sciences AG, Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria. Read user reviews, compare products & request pricing of BIOCRATES Life Sciences AG - ADME-Toxicology laboratory products & instrumentation. 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